Figure 3 : The run speed however is slow in linear, very slow in circular and the fastest run happens anti circular. This allows to re run the anticircular separation - after the plate has been dried completely. The spots turn from long to circular and the Rf values are after the second run exactly equal to those found in linear separations. Click again onto the right arrow above. Only problem with this fast elegant technique: when we started it (in the seventieth) there was no quantitation software available. Today we use a digital camera and the circular multi integration software from SORBFIL. Check the µ-PLC part of this internet book.